cd11b-pe cy7 m1 Search Results


rat-anti-mouse cd11b pe-cy7 m1/70  (Becton Dickinson)
90
Becton Dickinson rat-anti-mouse cd11b pe-cy7 m1/70
Rat Anti Mouse Cd11b Pe Cy7 M1/70, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b-pe+cy7+m1/anti+cd3/pm35587199-141-17-24
Average 90 stars, based on 1 article reviews
rat-anti-mouse cd11b pe-cy7 m1/70 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
rat anti-human cd47 b6h12  (Becton Dickinson)
90
Becton Dickinson rat anti-human cd47 b6h12
(A) Marrow macrophages were engineered and mixed with streptavidin beads of 900 nm to 6.7 μm diameter or else with disaggregated tdTom A549 tumors. Beads are opsonized with anti-streptavidin except for a non-targeted control in which SIRPα-inhibited macrophages were incubated with 2.1 μm lacking anti-streptavidin. These were not engulfed. After pre-incubation of the mixtures for up to 150 min, mixtures were plated for 24 hr on top of 5-μm pore transwells. Cells collected from transwell tops and bottoms were stained for <t>CD11b+,F4/80+</t> and analyzed by flow cytometry for engulfment (STAR Methods).
Rat Anti Human Cd47 B6h12, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b-pe+cy7+m1/anti+cd47/pmc05846676-593-38-44
Average 90 stars, based on 1 article reviews
rat anti-human cd47 b6h12 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
cd11b-pe-cy7  (Sony)
90
Sony cd11b-pe-cy7
(A) Marrow macrophages were engineered and mixed with streptavidin beads of 900 nm to 6.7 μm diameter or else with disaggregated tdTom A549 tumors. Beads are opsonized with anti-streptavidin except for a non-targeted control in which SIRPα-inhibited macrophages were incubated with 2.1 μm lacking anti-streptavidin. These were not engulfed. After pre-incubation of the mixtures for up to 150 min, mixtures were plated for 24 hr on top of 5-μm pore transwells. Cells collected from transwell tops and bottoms were stained for <t>CD11b+,F4/80+</t> and analyzed by flow cytometry for engulfment (STAR Methods).
Cd11b Pe Cy7, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b-pe+cy7+m1/anti+cd11b+antibody/pmc06359338-217-68-72
Average 90 stars, based on 1 article reviews
cd11b-pe-cy7 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
cd11b antibody / mac-1  (NSJ Bioreagents)
99
NSJ Bioreagents cd11b antibody / mac-1
(A) Marrow macrophages were engineered and mixed with streptavidin beads of 900 nm to 6.7 μm diameter or else with disaggregated tdTom A549 tumors. Beads are opsonized with anti-streptavidin except for a non-targeted control in which SIRPα-inhibited macrophages were incubated with 2.1 μm lacking anti-streptavidin. These were not engulfed. After pre-incubation of the mixtures for up to 150 min, mixtures were plated for 24 hr on top of 5-μm pore transwells. Cells collected from transwell tops and bottoms were stained for <t>CD11b+,F4/80+</t> and analyzed by flow cytometry for engulfment (STAR Methods).
Cd11b Antibody / Mac 1, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b-pe+cy7+m1/CD11b+Antibody+%2F+MAC-1/custom%40v2161%40pmc04846978__mmc1
Average 99 stars, based on 1 article reviews
cd11b antibody / mac-1 - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
cd11b pe cy7  (Cytek Biosciences)
93
Cytek Biosciences cd11b pe cy7
(A) The initial observation of the proportion of H2-A + cells among immune cells with broad myeloid markers. One experiment; a Student’s t test with Holm-Sidak’s multiple testing correction was used. (B) Representative flow plots illustrate the gating strategy used to identify P1–4 monocyte/macrophage cells. CD45 + , Lin − (CD3, NK1.1, Siglec-F, CD19), <t>CD11b</t> + , CD11c + cells are input. (C–E) Proportion (C and E) and MFI (D) of P1, P2, and P3/4 monocyte/macrophages (C and D) and DCs (D and E) under homeostatic SPF conditions in 9-week-old male and female mice. P3/4 are not shown for PP because they are not CD64 + . n = 6 WT and 5 IEC ΔMHCII . Data are representative of 2 experiments. A Student’s t test with Holm-Sidak’s multiple testing correction was used. (F) As in (C)–(E) but showing the CD11c-negative subset of P3/4 macrophages.
Cd11b Pe Cy7, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b-pe+cy7+m1/PE-Cyanine7+Anti-Human%2FMouse+CD11b/pmc09012449-24-0-5
Average 93 stars, based on 1 article reviews
cd11b pe cy7 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
cd3e, mouse(145-2c11)  (Biotium)
99
Biotium cd3e, mouse(145-2c11)
(A) The initial observation of the proportion of H2-A + cells among immune cells with broad myeloid markers. One experiment; a Student’s t test with Holm-Sidak’s multiple testing correction was used. (B) Representative flow plots illustrate the gating strategy used to identify P1–4 monocyte/macrophage cells. CD45 + , Lin − (CD3, NK1.1, Siglec-F, CD19), <t>CD11b</t> + , CD11c + cells are input. (C–E) Proportion (C and E) and MFI (D) of P1, P2, and P3/4 monocyte/macrophages (C and D) and DCs (D and E) under homeostatic SPF conditions in 9-week-old male and female mice. P3/4 are not shown for PP because they are not CD64 + . n = 6 WT and 5 IEC ΔMHCII . Data are representative of 2 experiments. A Student’s t test with Holm-Sidak’s multiple testing correction was used. (F) As in (C)–(E) but showing the CD11c-negative subset of P3/4 macrophages.
Cd3e, Mouse(145 2c11), supplied by Biotium, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b-pe+cy7+m1/CD3e%2C+Mouse(145-2C11)/custom%40bnc042013-100%40pmc05055382__1600972_SM
Average 99 stars, based on 1 article reviews
cd3e, mouse(145-2c11) - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
cd3 epsilon antibody  (NSJ Bioreagents)
99
NSJ Bioreagents cd3 epsilon antibody
(A) The initial observation of the proportion of H2-A + cells among immune cells with broad myeloid markers. One experiment; a Student’s t test with Holm-Sidak’s multiple testing correction was used. (B) Representative flow plots illustrate the gating strategy used to identify P1–4 monocyte/macrophage cells. CD45 + , Lin − (CD3, NK1.1, Siglec-F, CD19), <t>CD11b</t> + , CD11c + cells are input. (C–E) Proportion (C and E) and MFI (D) of P1, P2, and P3/4 monocyte/macrophages (C and D) and DCs (D and E) under homeostatic SPF conditions in 9-week-old male and female mice. P3/4 are not shown for PP because they are not CD64 + . n = 6 WT and 5 IEC ΔMHCII . Data are representative of 2 experiments. A Student’s t test with Holm-Sidak’s multiple testing correction was used. (F) As in (C)–(E) but showing the CD11c-negative subset of P3/4 macrophages.
Cd3 Epsilon Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b-pe+cy7+m1/CD3+epsilon+Antibody/custom%40v8271%40pmc05055382__1600972_SM
Average 99 stars, based on 1 article reviews
cd3 epsilon antibody - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
nk1.1 / cd161c / klrb1c, mouse(pk136)  (Biotium)
91
Biotium nk1.1 / cd161c / klrb1c, mouse(pk136)
(A) The initial observation of the proportion of H2-A + cells among immune cells with broad myeloid markers. One experiment; a Student’s t test with Holm-Sidak’s multiple testing correction was used. (B) Representative flow plots illustrate the gating strategy used to identify P1–4 monocyte/macrophage cells. CD45 + , Lin − (CD3, NK1.1, Siglec-F, CD19), <t>CD11b</t> + , CD11c + cells are input. (C–E) Proportion (C and E) and MFI (D) of P1, P2, and P3/4 monocyte/macrophages (C and D) and DCs (D and E) under homeostatic SPF conditions in 9-week-old male and female mice. P3/4 are not shown for PP because they are not CD64 + . n = 6 WT and 5 IEC ΔMHCII . Data are representative of 2 experiments. A Student’s t test with Holm-Sidak’s multiple testing correction was used. (F) As in (C)–(E) but showing the CD11c-negative subset of P3/4 macrophages.
Nk1.1 / Cd161c / Klrb1c, Mouse(pk136), supplied by Biotium, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b-pe+cy7+m1/NK1%2E1+%2F+CD161c+%2F+Klrb1c%2C+Mouse(PK136)/custom%40bnc042010-100%4041876981
Average 91 stars, based on 1 article reviews
nk1.1 / cd161c / klrb1c, mouse(pk136) - by Bioz Stars, 2026-09
91/100 stars
  Buy from Supplier
brdu (bromodeoxyuridine)(bu20a)  (Biotium)
99
Biotium brdu (bromodeoxyuridine)(bu20a)
(A) The initial observation of the proportion of H2-A + cells among immune cells with broad myeloid markers. One experiment; a Student’s t test with Holm-Sidak’s multiple testing correction was used. (B) Representative flow plots illustrate the gating strategy used to identify P1–4 monocyte/macrophage cells. CD45 + , Lin − (CD3, NK1.1, Siglec-F, CD19), <t>CD11b</t> + , CD11c + cells are input. (C–E) Proportion (C and E) and MFI (D) of P1, P2, and P3/4 monocyte/macrophages (C and D) and DCs (D and E) under homeostatic SPF conditions in 9-week-old male and female mice. P3/4 are not shown for PP because they are not CD64 + . n = 6 WT and 5 IEC ΔMHCII . Data are representative of 2 experiments. A Student’s t test with Holm-Sidak’s multiple testing correction was used. (F) As in (C)–(E) but showing the CD11c-negative subset of P3/4 macrophages.
Brdu (Bromodeoxyuridine)(Bu20a), supplied by Biotium, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b-pe+cy7+m1/BrdU+(Bromodeoxyuridine)(BU20a)/custom%40bnub0522-100%40pm37721423__es3c03980_si_001
Average 99 stars, based on 1 article reviews
brdu (bromodeoxyuridine)(bu20a) - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
cd11b pe  (Cytek Biosciences)
94
Cytek Biosciences cd11b pe
(A) The initial observation of the proportion of H2-A + cells among immune cells with broad myeloid markers. One experiment; a Student’s t test with Holm-Sidak’s multiple testing correction was used. (B) Representative flow plots illustrate the gating strategy used to identify P1–4 monocyte/macrophage cells. CD45 + , Lin − (CD3, NK1.1, Siglec-F, CD19), <t>CD11b</t> + , CD11c + cells are input. (C–E) Proportion (C and E) and MFI (D) of P1, P2, and P3/4 monocyte/macrophages (C and D) and DCs (D and E) under homeostatic SPF conditions in 9-week-old male and female mice. P3/4 are not shown for PP because they are not CD64 + . n = 6 WT and 5 IEC ΔMHCII . Data are representative of 2 experiments. A Student’s t test with Holm-Sidak’s multiple testing correction was used. (F) As in (C)–(E) but showing the CD11c-negative subset of P3/4 macrophages.
Cd11b Pe, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b-pe+cy7+m1/PE+Anti-Human%2FMouse+CD11b/pmc08983145-157-8-11
Average 94 stars, based on 1 article reviews
cd11b pe - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
cd11b-pe (m1/70)  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc cd11b-pe (m1/70)
(A) The initial observation of the proportion of H2-A + cells among immune cells with broad myeloid markers. One experiment; a Student’s t test with Holm-Sidak’s multiple testing correction was used. (B) Representative flow plots illustrate the gating strategy used to identify P1–4 monocyte/macrophage cells. CD45 + , Lin − (CD3, NK1.1, Siglec-F, CD19), <t>CD11b</t> + , CD11c + cells are input. (C–E) Proportion (C and E) and MFI (D) of P1, P2, and P3/4 monocyte/macrophages (C and D) and DCs (D and E) under homeostatic SPF conditions in 9-week-old male and female mice. P3/4 are not shown for PP because they are not CD64 + . n = 6 WT and 5 IEC ΔMHCII . Data are representative of 2 experiments. A Student’s t test with Holm-Sidak’s multiple testing correction was used. (F) As in (C)–(E) but showing the CD11c-negative subset of P3/4 macrophages.
Cd11b Pe (M1/70), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b-pe+cy7+m1/easysep+human+t+cell+isolation+kit/us11291674-627-59-62
Average 90 stars, based on 1 article reviews
cd11b-pe (m1/70) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
bromodeoxyuridine / brdu antibody  (NSJ Bioreagents)
99
NSJ Bioreagents bromodeoxyuridine / brdu antibody
(A) The initial observation of the proportion of H2-A + cells among immune cells with broad myeloid markers. One experiment; a Student’s t test with Holm-Sidak’s multiple testing correction was used. (B) Representative flow plots illustrate the gating strategy used to identify P1–4 monocyte/macrophage cells. CD45 + , Lin − (CD3, NK1.1, Siglec-F, CD19), <t>CD11b</t> + , CD11c + cells are input. (C–E) Proportion (C and E) and MFI (D) of P1, P2, and P3/4 monocyte/macrophages (C and D) and DCs (D and E) under homeostatic SPF conditions in 9-week-old male and female mice. P3/4 are not shown for PP because they are not CD64 + . n = 6 WT and 5 IEC ΔMHCII . Data are representative of 2 experiments. A Student’s t test with Holm-Sidak’s multiple testing correction was used. (F) As in (C)–(E) but showing the CD11c-negative subset of P3/4 macrophages.
Bromodeoxyuridine / Brdu Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd11b-pe+cy7+m1/Bromodeoxyuridine+%2F+BrdU+Antibody/custom%40v3127%40pm37721423__es3c03980_si_001
Average 99 stars, based on 1 article reviews
bromodeoxyuridine / brdu antibody - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

Image Search Results


(A) Marrow macrophages were engineered and mixed with streptavidin beads of 900 nm to 6.7 μm diameter or else with disaggregated tdTom A549 tumors. Beads are opsonized with anti-streptavidin except for a non-targeted control in which SIRPα-inhibited macrophages were incubated with 2.1 μm lacking anti-streptavidin. These were not engulfed. After pre-incubation of the mixtures for up to 150 min, mixtures were plated for 24 hr on top of 5-μm pore transwells. Cells collected from transwell tops and bottoms were stained for CD11b+,F4/80+ and analyzed by flow cytometry for engulfment (STAR Methods).

Journal: Current biology : CB

Article Title: SIRPA-Inhibited, Marrow-Derived Macrophages Engorge, Accumulate, and Differentiate in Antibody-Targeted Regression of Solid Tumors

doi: 10.1016/j.cub.2017.06.005

Figure Lengend Snippet: (A) Marrow macrophages were engineered and mixed with streptavidin beads of 900 nm to 6.7 μm diameter or else with disaggregated tdTom A549 tumors. Beads are opsonized with anti-streptavidin except for a non-targeted control in which SIRPα-inhibited macrophages were incubated with 2.1 μm lacking anti-streptavidin. These were not engulfed. After pre-incubation of the mixtures for up to 150 min, mixtures were plated for 24 hr on top of 5-μm pore transwells. Cells collected from transwell tops and bottoms were stained for CD11b+,F4/80+ and analyzed by flow cytometry for engulfment (STAR Methods).

Article Snippet: REAGENT OR RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human SIRPα (clone SE7C2) Santa Cruz sc-23863 Rat anti-mouse SIRPα (clone P84) BD Biosciences 552371 Rabbit anti-human RBCs (polyclonal) Rockland 109-4139 Anti-human CD41-FITC (HIP8) Biolegend 303703 Anti-human CD47-FITC (Clone CC2C6) Biolegend 323106 Rat Anti-human CD47 (Clone B6H12) BD Biosciences 556044 Anti-mouse CD11b-PE/Cy7 (Clone M1/70) Biolegend 101215 Anti-mouse F4/80-APC/Cy7 (clone BM8) Biolegend 123113 Donkey Anti-rabbit AF488 ThermoFisher A-21206 Donkey Anti-rabbit AF647 ThermoFisher A-31573 IgG from rabbit serum Sigma I5006-10MG Mouse anti-human Mucin (Clone S.854.6) ThermoFisher MA5-15131 Cetuximab InVivogen hegfr-mab1 Goat anti-rabbit AF700 ThermoFisher A-21038 Goat anti-rat AF647 ThermoFisher A-21247 Anti-mouse Ly-6G (Gr-1) APC (Clone RB6-8C5) Biolegend 108411 Anti-mouse CD135-BV421 (Clone A2F10.1) Biosciences 562898 Anti-mouse SIRPα-FITC (Clone P84) Biosciences 144005 Anti-human CD14-BV421 (Clone HCD14) Biolegend 325627 Anti-human CD33-APC (Clone WM53) Biolegend 303407 Anti-human CD66-PerCP/Cy5.5 (Clone G10F5) Biolegend 305107 Biological Samples Human Bone Marrow AllCells ABM001-1 NSG Mice The Jackson Laboratory 5557 Chemicals, Peptides, and Recombinant Proteins 7-Amino-actinomycin D Sigma A9400-1MG Hoechst 33342 ThermoFisher H3570 Phorbol myristate acetate Sigma P8139-1MG CFDA-SE ThermoFisher V12883 RBC Lysis Buffer Sigma R7757 Dispase StemCell Technologies 7913 Collagenase Sigma C0130-100MG DNase I Sigma 11284932001 Anti-streptavidin (polyclonal) Sigma S6390 Critical Commercial Assays F(Ab′)2 Preparation Kit ThermoFisher 44988 SIRPα ShRNA Lentiviral Plasmid Santa Cruz sc-44106-SH Streptavidin Beads ThermoFisher 11205D RNeasy plus Mini Kit QIAGEN 74134 TruSeq Stranded mRNA Library Prep kit Illumina RS-122-2101 Cell Lines Human adenocarcinoma (A549) ATCC CCL-185 Human Monocyte (THP-1) ATCC TIB-202 Primary liver carcinoma (EC4) Chi Van Dang’s Lab N/A Mesenchymal Stem Cells Progeria Research Foundation N/A Human hepatocellular carcinoma (HepG2) ATCC HB-8065 Mouse myoblast (C2C12) ATCC CRL-1772 Software MaxQuant Version 1.5.3.8 Max Planck Institute of Biochemistry http://www.biochem.mpg.de/5111795/maxquant Living Image Perkin Elmer http://www.perkinelmer.com/product/spectrum-200-living-image-v4series-1-128113 ImageJ NIH https://imagej.nih.gov/ij/ Open in a separate window KEY RESOURCES TABLE

Techniques: Incubation, Staining, Flow Cytometry

(A) Sirpa:Cd47 ratios increase in macrophages from microenvironments of increasing stiffness.

Journal: Current biology : CB

Article Title: SIRPA-Inhibited, Marrow-Derived Macrophages Engorge, Accumulate, and Differentiate in Antibody-Targeted Regression of Solid Tumors

doi: 10.1016/j.cub.2017.06.005

Figure Lengend Snippet: (A) Sirpa:Cd47 ratios increase in macrophages from microenvironments of increasing stiffness.

Article Snippet: REAGENT OR RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human SIRPα (clone SE7C2) Santa Cruz sc-23863 Rat anti-mouse SIRPα (clone P84) BD Biosciences 552371 Rabbit anti-human RBCs (polyclonal) Rockland 109-4139 Anti-human CD41-FITC (HIP8) Biolegend 303703 Anti-human CD47-FITC (Clone CC2C6) Biolegend 323106 Rat Anti-human CD47 (Clone B6H12) BD Biosciences 556044 Anti-mouse CD11b-PE/Cy7 (Clone M1/70) Biolegend 101215 Anti-mouse F4/80-APC/Cy7 (clone BM8) Biolegend 123113 Donkey Anti-rabbit AF488 ThermoFisher A-21206 Donkey Anti-rabbit AF647 ThermoFisher A-31573 IgG from rabbit serum Sigma I5006-10MG Mouse anti-human Mucin (Clone S.854.6) ThermoFisher MA5-15131 Cetuximab InVivogen hegfr-mab1 Goat anti-rabbit AF700 ThermoFisher A-21038 Goat anti-rat AF647 ThermoFisher A-21247 Anti-mouse Ly-6G (Gr-1) APC (Clone RB6-8C5) Biolegend 108411 Anti-mouse CD135-BV421 (Clone A2F10.1) Biosciences 562898 Anti-mouse SIRPα-FITC (Clone P84) Biosciences 144005 Anti-human CD14-BV421 (Clone HCD14) Biolegend 325627 Anti-human CD33-APC (Clone WM53) Biolegend 303407 Anti-human CD66-PerCP/Cy5.5 (Clone G10F5) Biolegend 305107 Biological Samples Human Bone Marrow AllCells ABM001-1 NSG Mice The Jackson Laboratory 5557 Chemicals, Peptides, and Recombinant Proteins 7-Amino-actinomycin D Sigma A9400-1MG Hoechst 33342 ThermoFisher H3570 Phorbol myristate acetate Sigma P8139-1MG CFDA-SE ThermoFisher V12883 RBC Lysis Buffer Sigma R7757 Dispase StemCell Technologies 7913 Collagenase Sigma C0130-100MG DNase I Sigma 11284932001 Anti-streptavidin (polyclonal) Sigma S6390 Critical Commercial Assays F(Ab′)2 Preparation Kit ThermoFisher 44988 SIRPα ShRNA Lentiviral Plasmid Santa Cruz sc-44106-SH Streptavidin Beads ThermoFisher 11205D RNeasy plus Mini Kit QIAGEN 74134 TruSeq Stranded mRNA Library Prep kit Illumina RS-122-2101 Cell Lines Human adenocarcinoma (A549) ATCC CCL-185 Human Monocyte (THP-1) ATCC TIB-202 Primary liver carcinoma (EC4) Chi Van Dang’s Lab N/A Mesenchymal Stem Cells Progeria Research Foundation N/A Human hepatocellular carcinoma (HepG2) ATCC HB-8065 Mouse myoblast (C2C12) ATCC CRL-1772 Software MaxQuant Version 1.5.3.8 Max Planck Institute of Biochemistry http://www.biochem.mpg.de/5111795/maxquant Living Image Perkin Elmer http://www.perkinelmer.com/product/spectrum-200-living-image-v4series-1-128113 ImageJ NIH https://imagej.nih.gov/ij/ Open in a separate window KEY RESOURCES TABLE

Techniques:

KEY RESOURCES TABLE

Journal: Current biology : CB

Article Title: SIRPA-Inhibited, Marrow-Derived Macrophages Engorge, Accumulate, and Differentiate in Antibody-Targeted Regression of Solid Tumors

doi: 10.1016/j.cub.2017.06.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT OR RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human SIRPα (clone SE7C2) Santa Cruz sc-23863 Rat anti-mouse SIRPα (clone P84) BD Biosciences 552371 Rabbit anti-human RBCs (polyclonal) Rockland 109-4139 Anti-human CD41-FITC (HIP8) Biolegend 303703 Anti-human CD47-FITC (Clone CC2C6) Biolegend 323106 Rat Anti-human CD47 (Clone B6H12) BD Biosciences 556044 Anti-mouse CD11b-PE/Cy7 (Clone M1/70) Biolegend 101215 Anti-mouse F4/80-APC/Cy7 (clone BM8) Biolegend 123113 Donkey Anti-rabbit AF488 ThermoFisher A-21206 Donkey Anti-rabbit AF647 ThermoFisher A-31573 IgG from rabbit serum Sigma I5006-10MG Mouse anti-human Mucin (Clone S.854.6) ThermoFisher MA5-15131 Cetuximab InVivogen hegfr-mab1 Goat anti-rabbit AF700 ThermoFisher A-21038 Goat anti-rat AF647 ThermoFisher A-21247 Anti-mouse Ly-6G (Gr-1) APC (Clone RB6-8C5) Biolegend 108411 Anti-mouse CD135-BV421 (Clone A2F10.1) Biosciences 562898 Anti-mouse SIRPα-FITC (Clone P84) Biosciences 144005 Anti-human CD14-BV421 (Clone HCD14) Biolegend 325627 Anti-human CD33-APC (Clone WM53) Biolegend 303407 Anti-human CD66-PerCP/Cy5.5 (Clone G10F5) Biolegend 305107 Biological Samples Human Bone Marrow AllCells ABM001-1 NSG Mice The Jackson Laboratory 5557 Chemicals, Peptides, and Recombinant Proteins 7-Amino-actinomycin D Sigma A9400-1MG Hoechst 33342 ThermoFisher H3570 Phorbol myristate acetate Sigma P8139-1MG CFDA-SE ThermoFisher V12883 RBC Lysis Buffer Sigma R7757 Dispase StemCell Technologies 7913 Collagenase Sigma C0130-100MG DNase I Sigma 11284932001 Anti-streptavidin (polyclonal) Sigma S6390 Critical Commercial Assays F(Ab′)2 Preparation Kit ThermoFisher 44988 SIRPα ShRNA Lentiviral Plasmid Santa Cruz sc-44106-SH Streptavidin Beads ThermoFisher 11205D RNeasy plus Mini Kit QIAGEN 74134 TruSeq Stranded mRNA Library Prep kit Illumina RS-122-2101 Cell Lines Human adenocarcinoma (A549) ATCC CCL-185 Human Monocyte (THP-1) ATCC TIB-202 Primary liver carcinoma (EC4) Chi Van Dang’s Lab N/A Mesenchymal Stem Cells Progeria Research Foundation N/A Human hepatocellular carcinoma (HepG2) ATCC HB-8065 Mouse myoblast (C2C12) ATCC CRL-1772 Software MaxQuant Version 1.5.3.8 Max Planck Institute of Biochemistry http://www.biochem.mpg.de/5111795/maxquant Living Image Perkin Elmer http://www.perkinelmer.com/product/spectrum-200-living-image-v4series-1-128113 ImageJ NIH https://imagej.nih.gov/ij/ Open in a separate window KEY RESOURCES TABLE

Techniques: Recombinant, Lysis, shRNA, Plasmid Preparation, Software

(A) The initial observation of the proportion of H2-A + cells among immune cells with broad myeloid markers. One experiment; a Student’s t test with Holm-Sidak’s multiple testing correction was used. (B) Representative flow plots illustrate the gating strategy used to identify P1–4 monocyte/macrophage cells. CD45 + , Lin − (CD3, NK1.1, Siglec-F, CD19), CD11b + , CD11c + cells are input. (C–E) Proportion (C and E) and MFI (D) of P1, P2, and P3/4 monocyte/macrophages (C and D) and DCs (D and E) under homeostatic SPF conditions in 9-week-old male and female mice. P3/4 are not shown for PP because they are not CD64 + . n = 6 WT and 5 IEC ΔMHCII . Data are representative of 2 experiments. A Student’s t test with Holm-Sidak’s multiple testing correction was used. (F) As in (C)–(E) but showing the CD11c-negative subset of P3/4 macrophages.

Journal: Cell reports

Article Title: Epithelial-myeloid exchange of MHC class II constrains immunity and microbiota composition

doi: 10.1016/j.celrep.2021.109916

Figure Lengend Snippet: (A) The initial observation of the proportion of H2-A + cells among immune cells with broad myeloid markers. One experiment; a Student’s t test with Holm-Sidak’s multiple testing correction was used. (B) Representative flow plots illustrate the gating strategy used to identify P1–4 monocyte/macrophage cells. CD45 + , Lin − (CD3, NK1.1, Siglec-F, CD19), CD11b + , CD11c + cells are input. (C–E) Proportion (C and E) and MFI (D) of P1, P2, and P3/4 monocyte/macrophages (C and D) and DCs (D and E) under homeostatic SPF conditions in 9-week-old male and female mice. P3/4 are not shown for PP because they are not CD64 + . n = 6 WT and 5 IEC ΔMHCII . Data are representative of 2 experiments. A Student’s t test with Holm-Sidak’s multiple testing correction was used. (F) As in (C)–(E) but showing the CD11c-negative subset of P3/4 macrophages.

Article Snippet: CD11b PE-Cy7, clone M1/70 , Tonbo Biosciences , Cat#60-0112; RRID: AB_2621836.

Techniques:

(A) The proportion of mT + mG + MNP subsets in the siLP. Cells subsets are pregated on Live, Dump − (CD3, CD19, NK1.1, Ly6G), CD45 + , and MHC class II + . Representative flow plots are shown for CD11c + macrophages and DCs. Two-way ANOVA with Holm-Sidak correction was used. (B) Representative image panel of cells from imaging flow cytometry analysis of mT + mG + MNPs. Cells are Live, Dump − (CD3, IgM), CD45 + , and MHC class II + . No CD11c or CD11b subsetting due to reduced channel availability on imaging cytometry platform was used. (C) A cartoon schematic of mixed MHC class II bone-marrow transfer experiments. (D) The percentage of host MHC class II (H2-A b ) positive donor cells (HLA-DR) in the siLP. Cells are CD11c − P3/P4 macrophages gated as in previous figures. A representative plot (pregated on CD45 + , Dump-, CD11b + /CD11c + , and CX3CR1 + /CD64 + cells) is shown, along with an overlay of the H2-A b isotype control demonstrating the MHC class II-haplotype specificity of the staining.

Journal: Cell reports

Article Title: Epithelial-myeloid exchange of MHC class II constrains immunity and microbiota composition

doi: 10.1016/j.celrep.2021.109916

Figure Lengend Snippet: (A) The proportion of mT + mG + MNP subsets in the siLP. Cells subsets are pregated on Live, Dump − (CD3, CD19, NK1.1, Ly6G), CD45 + , and MHC class II + . Representative flow plots are shown for CD11c + macrophages and DCs. Two-way ANOVA with Holm-Sidak correction was used. (B) Representative image panel of cells from imaging flow cytometry analysis of mT + mG + MNPs. Cells are Live, Dump − (CD3, IgM), CD45 + , and MHC class II + . No CD11c or CD11b subsetting due to reduced channel availability on imaging cytometry platform was used. (C) A cartoon schematic of mixed MHC class II bone-marrow transfer experiments. (D) The percentage of host MHC class II (H2-A b ) positive donor cells (HLA-DR) in the siLP. Cells are CD11c − P3/P4 macrophages gated as in previous figures. A representative plot (pregated on CD45 + , Dump-, CD11b + /CD11c + , and CX3CR1 + /CD64 + cells) is shown, along with an overlay of the H2-A b isotype control demonstrating the MHC class II-haplotype specificity of the staining.

Article Snippet: CD11b PE-Cy7, clone M1/70 , Tonbo Biosciences , Cat#60-0112; RRID: AB_2621836.

Techniques: Imaging, Flow Cytometry, Cytometry, Control, Staining

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Epithelial-myeloid exchange of MHC class II constrains immunity and microbiota composition

doi: 10.1016/j.celrep.2021.109916

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: CD11b PE-Cy7, clone M1/70 , Tonbo Biosciences , Cat#60-0112; RRID: AB_2621836.

Techniques: Virus, Recombinant, SYBR Green Assay, Cell Isolation, DNA Extraction, Enzyme-linked Immunosorbent Assay, Sequencing, Expressing, Software, Luminex